Ajọ Fluorescence jẹ paati bọtini ti a lo ninu awọn ohun elo imọ-jinlẹ ati igbesi aye.Iṣe akọkọ rẹ ni lati yapa ati yan irisi oju-iwe gigun abuda lati ina inudidun ati itanna itujade ti nkan na ni ayewo fluorescence biomedical ati eto itupalẹ.Awọn asẹ Fluorescence jẹ afihan deede nipasẹ ijinle gige-pipa ti o jinlẹ ati kekere autofluorescence.Nigbagbogbo, awọn asẹ lọpọlọpọ le jẹ lẹ pọ lati ṣe àlẹmọ fluorescence kan, eyiti o ni iṣẹ ṣiṣe idiyele giga.
Ajọ Fluorescence jẹ lilo ni ohun elo pipo fluorescence gidi-akoko PCR.eyiti o jẹ lilo pupọ ni isedale molikula ati wiwa aabo ounje ati abojuto ajakale-arun ilera gbogbogbo ati awọn ile-iṣẹ miiran.
Ajọ Fluorescence ni a lo fun wiwa ọpọlọpọ awọn awọ ati awọn iwadii, fun apẹẹrẹ:
FAM / SYBR Green / Green / HEX / TET / Cy3 / JOE / ROX / Cy3.5 / Texas Red, Cy5 / LC Red640, Cy5.5 ati be be lo
Ilana | (IAD Aso Lile) | ||
Igi gigun | Ex (nm) | Emu(nm) | Agbelebu |
| 470-30 | 525-20 | > 6 |
| 523-20 | 564-20 | > 6 |
| 543-20 | 584-20 | > 6 |
| 571-20 | 612-20 | > 6 |
| 628-35 | 692-45 | > 6 |
Ìdènà | OD>6@200~900nm tabi @200~1200nm | ||
Ite(nm) | 50 % ~ OD5 <10nm | ||
Agbelebu | OD>6 | ||
Iwọn (mm) | Φ4mm, Φ12mm, Φ12.7mm, Φ25.4mm ati be be lo |